Biochemical biosynthetic characterization cloning cluster cyanobacterium dissertation from gene hec

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Genetic and Biochemical Studies of Photosystem II Assembly

PMID: 12039053;Abstract: A genomic DNA region of over 80 kb that contains the complete biosynthetic gene cluster for the synthesis of the antifungal polyketide metabolite soraphen A was cloned from Sorangium cellulosum So ce26. The nucleotide sequence of the soraphen A gene region, including 67,523 bp was determined.

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Environmental conditions that influence toxin biosynthesis

Feb 15, 2019 · Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. J Nat Prod 70 : 1977 – 1986 . doi: 10.1021/np0704250 . OpenUrl CrossRef PubMed

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Cloning and functional characterization of carotenoid

Aug 26, 2008 · Hoffmann D, Hevel JM, Moore RE, Moore BS. Sequence analysis and biochemical characterization of the nostopeptolide A biosynthetic gene cluster from Nostoc sp. GSV224. Gene. 2003; 311:171–180. doi: 10.1016/S0378-1119(03)00587-0. Linne U, Doekel S, Marahiel MA.

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Evolution of Arginine Biosynthesis in the Bacterial Domain

Drug resistant infectious diseases are quickly becoming a global health crisis. While Streptomyces spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 Streptomyes spp. Using this methodology, we discovered

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PNAS Plus: Metagenomic natural product discovery in lichen

Cloning and Biochemical Characterization of the Hectochiorin Biosynthetic Gene Cluster from the Marine Cyanobacterium Lyngbya majuscula by Aishwarya V. Ramaswamy A DISSERTATION submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Presented June 02, 2005 Commencement June 2006

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Sucrose metabolism: Anabaena sucrose‐phosphate synthase

The interaction between photosystem I (PS I) and flavodoxin from the cyanobacterium Synechococcus sp. PCC 7002 was investigated by covalent cross‐linking in the presence of a hydrophilic crosslinker, N ‐ethyl‐3‐(3‐diaminopropyl)carbodiimide. Under the experimental conditions employed, five distinct cross‐linking products of flavodoxin and PS I subunits are formed.

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Glycolipid composition of the heterocyst envelope of

Cloning, sequencing, and biochemical characterization of the nostocyclopeptide biosynthetic gene cluster: Molecular basis for imine macrocyclization February 2004 Gene 325(1):35-42

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Single Cell Genome Amplification Accelerates

Irene Saugar, Brian Molloy, Eloisa Sanz, María Blanca Sánchez, María Fernández-Lobato, Antonio Jiménez, Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus, The Journal of Antibiotics, 10.1038/ja.2016.123, 70, 4, (404-413), (2016).

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Graduate Thesis Or Dissertation | Cloning and biochemical

Ramaswamy AV, Sorrels CM, Gerwick WH. Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. J. Nat. Prod. 2007; 70:1977–1986.

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Metabolic Engineering of Synechocystis sp. PCC 6803 for

Apr 01, 2016 · The biosynthesis pathway for CYN has been partially elucidated by isotope-labelled precursor feeding experiments (Burgoyne et al., 2000) and subsequent bioinformatic characterization of the cyr (or aoa) gene cluster from Cylindrospermopsis raciborskii AWT205 (Shalev-Alon et al., 2002, Mihali et al., 2008).

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Overproduction and easy recovery of biofuels from

Jun 01, 2006 · Furthermore, identification of the biosynthetic genes and elucidation of the biosynthesis pathway is a prerequisite for improving the production through expression of the biosynthetic gene cluster in heterologous host organisms 28, 30, 31. It is also the essential first step for efforts to increase molecular diversity through combinatorial

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Phage auxiliary metabolic genes and the redirection of

Jun 05, 2003 · The cloning, sequencing, annotation and biochemical analysis of the nostopeptolide (nos) biosynthetic gene cluster from the terrestrial cyanobacterium Nostoc sp. GSV224 is described. Nostopeptolides A1 and A2 are cyclic peptide-polyketide hybrid natural products possessing nine amino acid residues, a butyric acid group, and an internal acetate-derived unit that …

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Biosynthesis of marine toxins - ScienceDirect

Sep 27, 2011 · Cyanophages infecting the marine cyanobacteria Prochlorococcus and Synechococcus encode and express genes for the photosynthetic light reactions. Sequenced cyanophage genomes lack Calvin cycle genes, however, suggesting that photosynthetic energy harvested via phage proteins is not used for carbon fixation. We report here that cyanophages carry and express a Calvin cycle inhibitor, …

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Cloning and Biochemical Characterization of the

Aug 16, 2007 · Histidine biosynthesis is one of the best characterized anabolic pathways. There is a large body of genetic and biochemical information available, including operon structure, gene expression, and increasingly larger sequence databases. For over forty years this pathway has been the subject of extensive studies, mainly in Escherichia coli and Salmonella enterica, in both of which …

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Istvan Molnar | UA Profiles

divergently transcribed to the scytonemin gene cluster in N. punctiforme (Npun_F1277 and Npun_F1278). This TCRS is highly conserved in ori-entation and in its location upstream of the scytone-min gene cluster in at least 11 strains of cyanobacteria containing the putative scytonemin biosynthetic clusters, leading to the hypothesis that

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AN ABSTRACT OF THE DISSERTATION OF Redacted for privacy

Sep 04, 2007 · A metabolic pathway for biosynthesis of the nonreducing disaccharide mannosylfructose (β-fructofuranosyl-α-mannopyranoside), an important osmolyte in Agrobacterium tumefaciens , was discovered. We have identified and functionally characterized two ORFs that correspond to genes (named mfpsA and mfppA ) encoding the rare enzymes mannosylfructose-phosphate synthase and …

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Detection of nonribosomal peptide synthetase genes in

Mar 18, 2002 · Based on the functional characterization of sucrose biosynthesis related proteins [SBP: sucrose‐phosphate synthase (SPS), sucrose‐phosphate phosphatase (SPP), and sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture.

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Functional Genomic Analysis of the HY2 Family of

Mar 20, 2012 · The saxitoxin biosynthetic gene cluster has been reported in five genera of cyanobacteria (Kellmann et al., 2008; Mihali et al., 2009; 2011; Soto‐Liebe et al., 2010; Stucken et al., 2010). These gene clusters range in size from 25.7 kb in Raphidiopsis brookii D9 to 36 kb in Lyngbya wollei and consist of up to 33 genes encoding biosynthetic

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Cory Knoot - Senior Scientist - VaxNewMo, LLC | LinkedIn

Iwaki et al., "Cloning and Characterization of a Gene Cluster Involved in Cyclopentanol Metabolism in Comamonas sp. Strain NCIMB 9872 and Biotransformations Effected by Escherichia coli-Expressed Cyclopentanone 1,2-Monooxygenase," Appl Environ Microbiol., 2002, 68(11):5671-5684, 14 pages. (APP)

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Cohesion Group Approach for Evolutionary Analysis of

The evolutionary history was inferred using the Neighbor-Joining method [65]. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed [66]. Branches corresponding to partitions reproduced in less …

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Structure and Biosynthesis of the Antibiotic Bottromycin D

A comparison of flanking regions showed that our clone was located in the hopanoid biosynthesis gene cluster, as described previously (34, 46). tll1096 from T. elongatus BP-1 has 1,080 bp and encodes a 359-amino-acid protein (∼39.9 kDa). The open reading frame (ORF) was cloned by PCR using T. elongatus BP-1 genomic DNA as a template. Initial

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Biosynthesis of the ansamycin antibiotic rifamycin

SUMMARY DNA-binding repressor proteins that govern transcription initiation in response to end products generally regulate bacterial biosynthetic genes, but this is rarely true for the pyrimidine biosynthetic ( pyr ) genes. Instead, bacterial pyr gene regulation generally involves mechanisms that rely only on regulatory sequences embedded in the leader region of the operon, which cause

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Cloning, sequencing, and biochemical characterization of

Methanococcus maripaludis is a strictly anaerobic, methane-producing archaeon. It is a mesophile that utilizes CO 2 as the sole carbon source during autotrophic growth in minimal medium but assimilates acetate and some amino acids as carbon sources when they are present (28, 46, 47, 66).Regardless of the carbon source, the reduction of carbon dioxide to methane by H 2 or formate is a required

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(PDF) Characterization of the Gene Cluster Responsible for

Sequence analysis of the region bordering the hrp/dsp gene cluster of Erwinia amylovora strain Ea321, which causes fire blight, revealed characteristics of pathogenicity islands (PAIs). Included are genes for a phage integrase, a tRNA Phe, several orthologues of genes of YAPI, a PAI of Yersinia pseudotuberculosis, and several putative virulence genes with HrpL‐dependent promoter motifs.

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Biochemical and Mutational Analysis of Glutamine

If you want a customer helpline executives are as shown in the this is the biochemical biosynthetic characterization cloning cluster cyanobacterium dissertation from gene hec To take into your academic writing partner and get the best while still getting excellent work performed, they are academic goals youâve set. Read on; youll learn to

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(PDF) Natural Product Biosynthetic Diversity and

Feb 01, 1998 · Cloning of the gene encoding this enzyme, HfK, and demonstration that this gene is essential for rifamycin pro- duction [10] then provided a probe that allowed the isola- tion of the rifamycin biosynthetic gene cluster. The sequence and functional analysis of this cluster is …

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Recombination and selectional forces in cyanopeptolin NRPS

characterization of one of the isolated mutant strains that is lacking the gene RBD1 and, as a direct consequence, is defective in the assembly of PSII. In chapter 3, I present a biochemical characterization of the RBD1 protein. Chapter 4 contains the results of experiments on a double

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Screening of putative oxygenase genes in the Fusarium

Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2012.06 Daniel Ritz, Linda Th ny-Meyer, Hauke Hennecke, The cycHJKL gene cluster plays an essential role in the biogenesis of c-type cytochromes in Bradyrhizobium japonicum

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Two Biosynthetic Pathways for Aromatic Amino Acids in the

Tracing back the evolution of a metabolic pathway becomes possible when differences in biochemical characters and gene organization can be compared with a phylogenetic progression of the host organisms ().By applying this rationale to the different branches of the aromatic amino acid biosynthetic pathway, a paradigm was generated to study the molecular evolution of metabolism among Bacteria (1

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Sequence analysis and biochemical characterization of the

Thesis: “Secondary metabolism of basidiomycetes – Molecular biological and biochemical investigations on Paxillus involutus and Suillus grevillei” Advisor: Professor Dirk Hoffmeister Main Outcomes characterization of NRPS-like enzymes involved in the biosynthesis of the …

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Cloning, Solubilization, and Characterization of Squalene

As these biosynthetic pathways yield such similar natural products, it is possible that one gene cluster was derived from the other through the gain or loss of an alanine NRPS module. This event could have occurred in O. spongeliae in the recent past and would result in the chemical diversity observed in these cyanobacteria.

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Douglas Gatte Picchi - Analista acadêmico - Wyden

Diverse hydrocarbon biosynthetic enzymes can substitute for olefin synthase in the cyanobacterium Synechococcus sp. PCC 7002 Scientific Reports February 4, 2019

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A metabolic pathway leading to mannosylfructose

Aeruginoguanidine biosynthetic gene cluster has been only partially identified in the original producer strain M. aeruginosa Nies98 (unpublished data). Therefore, the objective of this study includes the complete sequencing of the missing agd biosynthetic gene cluster and the further in silico characterisation of the enzymes encoded in the cluster.

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Methods of producing 6-carbon chemicals via methyl-ester

Aug 13, 2013 · Two conventional cis-AT PKS–NRPS biosynthetic pathways have recently been described from cyanobacteria associated with lichens: the mcy gene cluster (35, 36) involved in synthesis of microcystins, notorious hepatotoxins typical of many cyanobacteria, and the crp gene cluster, responsible for production of cryptophycins , anticancer agents of more limited distribution.

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(PDF) Molecular cloning and expression in Escherichia coli

The HY2-Related Gene Family in Cyanobacteria, Oxyphotobacteria, and Plants. A recently published paper describes the cloning of the HY2 gene of Arabidopsis (Kohchi et al., 2001).Using the deduced protein sequence of HY2, TBLASTN, BLASTP, and PSI-BLAST searches (Altschul et al., 1990, 1997) were performed to identify putative bilin reductases in the nonredundant National Center for

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Interaction between Photosystem I and Flavodoxin from the

Two different genes encoding glutamine synthetase type I (GSI) and GSIII were identified in the genome sequence of R. albus 8. The identity of the GSIII protein was confirmed by the presence of its associated conserved motifs. The glnN gene, encoding the GSIII, was cloned and expressed in Escherichia coli BL21 cells. The recombinant protein was purified and subjected to biochemical and